fractogel tmae m prepacked column  (Merck & Co)

Journal: bioRxiv

Article Title: IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG

doi: 10.64898/2026.02.26.708374

Figure Lengend Snippet:

Article Snippet: A 5 mL Fractogel TMAE (M) prepacked column (MiniChrom format; Merck), was equilibrated with 25 mM sodium acetate buffer (pH 5.9).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG

doi: 10.64898/2026.02.26.708374

Figure Lengend Snippet: UV280 breakthrough chromatogram during anion-exchange chromatography on Fractogel TMAE (M) used to deplete IgA and IgM from the caprylic acid–purified EQ-IgG intermediate. The IgG-rich flow-through (breakthrough zone) was collected, whereas retained proteins (including IgA/IgM) were subsequently eluted during the regeneration step. The final peak corresponds to the column cleaning-in-place (CIP) step. UV absorbance is shown at 280 nm as a function of elution volume (mL).

Article Snippet: A 5 mL Fractogel TMAE (M) prepacked column (MiniChrom format; Merck), was equilibrated with 25 mM sodium acetate buffer (pH 5.9).

Techniques: Chromatography, Purification

Journal: bioRxiv

Article Title: IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG

doi: 10.64898/2026.02.26.708374

Figure Lengend Snippet: Effect of IgA/IgM depletion on Planova nanofiltration performance of caprylic acid–purified IgG (CA-IgG). (A) Direct nanofiltration of CA-IgG using Planova 20N (membrane area 0.001 m²) shows an early decline in permeate flux after ∼29 min, yielding only ∼16 mL of filtrate after 120 min (0.8 kgf/cm²). (B) Sequential filtration of CA-IgG using Planova 35N followed by 20N (0.001 m² each) under identical conditions does not prevent early flux decline (after ∼26–27 min) and results in a similar filtrate volume (∼15–16 mL after 120 min). (C) After Fractogel TMAE chromatography (IgA/IgM-depleted flow-through), sequential Planova 35N→20N filtration shows stable filtrate accumulation, indicating improved filterability after upstream removal of fouling components. (D,E) Volume–time plots used to derive permeate flow rates for the Fractogel TMAE flow-through during sequential filtration using Planova 35N→20N (D) and Planova 35N→S20N (E). Under the same feed conditions, the S20N run shows a higher steady permeate flow (∼1.5 mL/min) than the 20N run (∼0.5 mL/min). In panels A–C, cumulative filtrate mass (g) is plotted versus time; overlaid traces represent repeated runs.

Article Snippet: A 5 mL Fractogel TMAE (M) prepacked column (MiniChrom format; Merck), was equilibrated with 25 mM sodium acetate buffer (pH 5.9).

Techniques: Purification, Membrane, Filtration, Chromatography

Journal: bioRxiv

Article Title: IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG

doi: 10.64898/2026.02.26.708374

Figure Lengend Snippet: DLS and NTA profiles of the apparent size distribution and subvisible particulate content of IgG intermediates after Fractogel TMAE chromatography and Planova nanofiltration. (A) Dynamic light scattering (DLS) intensity-weighted hydrodynamic size distributions of the Fractogel flow-through and of products after Planova 35N filtration and sequential Planova 35N→20N filtration. Z-average hydrodynamic diameter and polydispersity index (PDI) are shown below each plot. (B) Representative DLS intensity distributions for the corresponding products (Fractogel–35N, Fractogel–35N–20N, and Fractogel–35N–S20N). (C) Nanoparticle tracking analysis (NTA) of the same IgG solutions showing the concentration and apparent size distribution of subvisible, light-scattering particles (IgG aggregates/particulates); total particle concentration (particles/mL) and measurements at comparable tracking density or particles per frame (∼30–33) are indicated in each panel. The NTA data show a marked reduction in detectable subvisible particles after Planova 20N or S20N filtration.

Article Snippet: A 5 mL Fractogel TMAE (M) prepacked column (MiniChrom format; Merck), was equilibrated with 25 mM sodium acetate buffer (pH 5.9).

Techniques: Chromatography, Filtration, Concentration Assay

Journal: bioRxiv

Article Title: IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG

doi: 10.64898/2026.02.26.708374

Figure Lengend Snippet: SDS–PAGE profile of IgG intermediates under non-reducing (conditions before and after IgA/IgM depletion and Planova nanofiltration. All samples show a dominant band at ∼150 kDa consistent with intact IgG and depletion of higher-molecular-weight species after sequential Planova nanofiltration. Lane assignments: (1) caprylic acid–purified IgG (CA-IgG); (2) Fractogel TMAE (M) flow-through (EQ-IgG); (3) Planova 35N filtrate; (4) sequential Planova 35N→20N filtrate; (5) sequential Planova 35N→S20N filtrate. Molecular weight markers (kDa) are shown on the left.

Article Snippet: A 5 mL Fractogel TMAE (M) prepacked column (MiniChrom format; Merck), was equilibrated with 25 mM sodium acetate buffer (pH 5.9).

Techniques: SDS Page, Molecular Weight, Purification